DNMT1 antibody (AA 1-270)

Catalog No. ABIN6139745

This Rabbit Polyclonal antibody specifically detects DNMT1 in WB, IP and IF. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for DNMT1 antibody (AA 1-270) (ABIN6139745)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-DNMT1 Antibody

Binding Specificity: AA 1-270

Sequence: MPARTAPARV PTLAVPAISL PDDVRRRLKD LERDSLTEKE CVKEKLNLLH EFLQTEIKNQ LCDLETKLRK EELSEEGYLA KVKSLLNKDL SLENGAHAYN REVNGRLENG NQARSEARRV GMADANSPPK PLSKPRTPRR SKSDGEAKPE PSPSPRITRK STRQTTITSH FAKGPAKRKP QEESERAKSD ESIKEEDKDQ DEKRRRVTSR ERVARPLPAE EPERAKSGTR TEKEEERDEK EEKRLRSQTK EPTPKQKLKE EPDREARAGV

Cross-Reactivity: Human

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IF,1:50 - 1:200,IP,1:50 - 1:100

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-DNMT1 antibody (ABIN6139745)

Xie, Zhang, Wang, Zhao, Zhang, Yao, Hur, Yeh, Pang, Zheng, Fan, Kong, Wang, Chiu, Zhou: "Matrix stiffness determines the phenotype of vascular smooth muscle cell in vitro and in vivo: Role of DNA methyltransferase 1." in: Biomaterials, Vol. 155, pp. 203-216, (2018) (PubMed).

Wang, Li, Zhu, Lin, Luo, Zhao, Zhang, Li, Gao, Liang, Liu, Tsun, Yuan, Wu, Li: "DNMT1 cooperates with MBD4 to inhibit the expression of Glucocorticoid-induced TNFR-related protein in human T cells." in: FEBS letters, Vol. 591, Issue 13, pp. 1929-1939, (2017) (PubMed).

Xi, Gao, Yang, Ye, Zhang, Wu, Jiang, Zhang: "Anticancer drugs induce hypomethylation of the acetylcholinesterase promoter via a phosphorylated-p38-DNMT1-AChE pathway in apoptotic hepatocellular carcinoma cells." in: The international journal of biochemistry & cell biology, Vol. 68, pp. 21-32, (2016) (PubMed).

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1

Background: This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.,ADCADN,AIM,CXXC9,DNMT,HSN1E,MCMT,m.HsaI,DNMT1,Epigenetics & Nuclear Signaling,Chromatin Modifying Enzymes,DNA methylation,DNMT1

Molecular Weight: 144 kDa/183 kDa/184 kDa

Gene ID: 1786

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection