MCM4 antibody (AA 350-450)

Catalog No. ABIN6143642

The Rabbit Polyclonal anti-MCM4 antibody has been validated for WB and IHC. It is suitable to detect MCM4 in samples from Human.

Quick Overview for MCM4 antibody (AA 350-450) (ABIN6143642)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MCM4 Antibody

Binding Specificity: AA 350-450

Sequence: QMIKLQESPE DMPAGQTPHT VILFAHNDLV DKVQPGDRVN VTGIYRAVPI RVNPRVSNVK SVYKTHIDVI HYRKTDAKRL HGLDEEAEQK LFSEKRVELL K

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MCM4 (NP_877423.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:100 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Alternative Name: MCM4

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.,MCM4,CDC21,CDC54,NKCD,NKGCD,P1-CDC21,hCdc21,Epigenetics & Nuclear Signaling,MCM4

Molecular Weight: 96 kDa

Gene ID: 4173

UniProt: P33991

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA