ROCK1 antibody (AA 400-660)

Catalog No. ABIN6147066

The Rabbit Polyclonal anti-ROCK1 antibody has been validated for WB, IHC and IP. It is suitable to detect ROCK1 in samples from Human.

Quick Overview for ROCK1 antibody (AA 400-660) (ABIN6147066)

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-ROCK1 Antibody

Binding Specificity: AA 400-660

Sequence: YSNRRYLSSA NPNDNRTSSN ADKSLQESLQ KTIYKLEEQL HNEMQLKDEM EQKCRTSNIK LDKIMKELDE EGNQRRNLES TVSQIEKEKM LLQHRINEYQ RKAEQENEKR RNVENEVSTL KDQLEDLKKV SQNSQLANEK LSQLQKQLEE ANDLLRTESD TAVRLRKSHT EMSKSISQLE SLNRELQERN RILENSKSQT DKDYYQLQAI LEAERRDRGH DSEMIGDLQA RITSLQEEVK HLKHNLEKVE GERKEAQDML N

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:100,IP,1:50 - 1:100

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for ROCK1

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Alternative Name: ROCK1

Background: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.,ROCK1,P160ROCK,ROCK-I,Cancer,Signal Transduction,G protein signaling,Kinase,Serine/threonine kinases,Cell Biology & Developmental Biology,Apoptosis,Cell Cycle,Centrosome,Cell Adhesion,Cytoskeleton,Microfilaments,Microtubules,Actins,Death Receptor Signaling Pathway,TGF-b-Smad Signaling Pathway,ROCK1

Molecular Weight: 158 kDa

Gene ID: 6093

UniProt: Q13464

Pathways: Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling