CEACAM1 antibody

Catalog No. ABIN614760

The Mouse Monoclonal anti-CEACAM1 antibody (Clone B3) (ABIN614760) specifically detects CEACAM1 in WB, FACS, IP, IF, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for CEACAM1 antibody (ABIN614760)

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: B3

Product Details anti-CEACAM1 Antibody

Specificity: This Monoclonal CEACAM-1 antibody (B3) interacts solely with Human CEACAM-1 and shows no cross-reactivity with other CEACAMs expressed in Human or other species! Does not react with Rat, Mouse, Dog and Cattle.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G Chromatography

Immunogen: Recombinant Human soluble CEACAM-1-Fc lacking the N-domain (produced in HEK293 cells)

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 100 μL sterile water corresponding to a concentration of 1.0 mg/mL.

Buffer: PBS, pH 7.4

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CEACAM1

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Alternative Name: CD66a / CEACAM1

Background: Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM1, also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I trans-membrane proteins (CEACAM1, 3-4) and GPI-linked molecules (CEACAM5-8). There is no CEACAM2 in human. The gene for human CEACAM1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type followed by three C2-type Ig-like domains. It shows considerable glycosylation (1). There are three soluble and seven transmembrane isoforms. The three soluble forms also contain the first two C2-type Ig like domains (aa's 145 317), with differences coming in the third C2-type Ig- like domain (6). The seven transmembrane isoforms are highly divergent. Full-length mouse and rat CEACAM1 are approximately 57 % aa identical to human CEACAM1, in the V-type Ig-like domain, they are 58 % and 56 % aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium.Synonyms: BGP, BGP1, Biliary glycoprotein 1, Carcinoembryonic antigen-related cell adhesion molecule 1

Gene ID: 634

NCBI Accession: NP_001703

UniProt: P13688