LGALS1/Galectin 1 antibody (AA 12-108) (CF®594)
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- Target See all LGALS1/Galectin 1 (LGALS1) Antibodies
- LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
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Binding Specificity
- AA 12-108
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This LGALS1/Galectin 1 antibody is conjugated to CF®594
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)
- Purpose
- Mouse Monoclonal anti-Galectin-1 / Human Placental Lactogen (GAL1/1831), CF594 Conjugate
- Characteristics
- Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 Molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human Galectin-1 protein fragment (aa12-108) (exact sequence is proprietary)
- Clone
- GAL1-1831
- Isotype
- IgG Mix lambda
- Top Product
- Discover our top product LGALS1 Primary Antibody
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- Application Notes
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Flow cytometry 0.5-1 μg/million cells in 0.1 mL
- Immunohistology (formalin) 0.5-1 μg/mL
- ELISA 2-4 μg/mL for coating (order Ab without BSA)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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HeLa, K562 or 293 cells. Prostate, Kidney, Placenta, Stomach, Skin, Spleen, Brain or Heart.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
- Alternative Name
- Galectin-1 / Placental Lactogen (LGALS1 Products)
- Synonyms
- GAL1 antibody, GBP antibody, Galectin-1 antibody, LGAS1 antibody, LGALS1 antibody, MGC64502 antibody, GB10026 antibody, AA410090 antibody, Gal-1 antibody, Galbp antibody, L-14.5 antibody, L14 antibody, Lect14 antibody, galectin-1 antibody, Galaptin antibody, galectin 1 antibody, galectin-1 antibody, lectin, galactoside-binding, soluble, 1 L homeolog antibody, protein enabled antibody, lectin, galactose binding, soluble 1 antibody, LGALS1 antibody, Lgals1 antibody, CC1G_05003 antibody, CC1G_05004 antibody, lgals1.L antibody, LOC408848 antibody
- Molecular Weight
- 14 kDa
- Gene ID
- 3956, 445351
- UniProt
- P09382
- Pathways
- Carbohydrate Homeostasis
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