HLA-DRA antibody (CF®405S)

Catalog No. ABIN6153377

This anti-HLA-DRA antibody is a Mouse Monoclonal antibody detecting HLA-DRA in FACS and IF. Suitable for Human, Cow and Cat.

Quick Overview for HLA-DRA antibody (CF®405S) (ABIN6153377)

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Reactivity: Human, Cow, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DRA antibody is conjugated to CF®405S

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 169-1B5-2

Product Details anti-HLA-DRA Antibody (CF®405S)

Purpose: Mouse Monoclonal anti-MHC-II (HLA-DRA) (169-1B5.2), CF405S Conjugate

Characteristics: This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4 T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human peripheral blood leukocytes

Isotype: IgG2b kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Ramos, Daudi or HuT78 cells. Tonsil or lymph node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for HLA-DRA

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Alternative Name: MHC-II (HLA-DRA)

Molecular Weight: 36 kDa (? chain) and 27 kDa (? chain)

Gene ID: 3122, 520048

UniProt: P01903

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Human Leukocyte Antigen (HLA) in Adaptive Immune Response