HLA-DRA antibody (CF®405S)

Catalog No. ABIN6153453

This Mouse Monoclonal antibody specifically detects HLA-DRA in FACS and IF. It exhibits reactivity toward Human and Monkey.

Quick Overview for HLA-DRA antibody (CF®405S) (ABIN6153453)

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DRA antibody is conjugated to CF®405S

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: IPO-10

Product Details anti-HLA-DRA Antibody (CF®405S)

Purpose: Mouse Monoclonal anti-MHC-II (HLA-DRA) (IPO-10), CF405S Conjugate

Characteristics: MAb IPO-10 defines the antigen, which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50 % of cells). The MAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This MAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70 % of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkin's disease cases a significant percentage of affected lymph node cells were detected. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Spleen cells of a patient with hairy cell leukemia (Daudi cells)

Isotype: IgG3 kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Ramos, Daudi or HuT78 cells. Tonsil or lymph node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for HLA-DRA

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Alternative Name: MHC-II (HLA-DRA)

Molecular Weight: 36 kDa

Gene ID: 3122, 520048

UniProt: P01903

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Human Leukocyte Antigen (HLA) in Adaptive Immune Response