Recombinant Melanoma gp100 antibody (CF®640R)

Catalog No. ABIN6154749

This Rabbit Monoclonal antibody specifically detects Melanoma gp100 in IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant Melanoma gp100 antibody (CF®640R) (ABIN6154749)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is conjugated to CF®640R

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PMEL-1825R

Product Details anti-Melanoma gp100 Antibody (CF®640R)

Purpose: Recombinant Rabbit Monoclonal anti-gp100 / Melanosome / PMEL17 / SILV (PMEL/1825R), CF640R Conjugate

Characteristics: Cytotoxic T lymphocytes (CTLs) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human SILV protein

Isotype: IgG kappa

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

SK-MEL-28 cells or Melanoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: gp100 / Melanosome / PMEL17 / SILV

Molecular Weight: 90-100 kDa

Gene ID: 6490, 95972

UniProt: P40967