CD80 antibody (CF®594)

Catalog No. ABIN6156555

This Mouse Monoclonal antibody specifically detects CD80 in FACS, ELISA, IF and Coat. It exhibits reactivity toward Human.

Quick Overview for CD80 antibody (CF®594) (ABIN6156555)

Target: CD80

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD80 antibody is conjugated to CF®594

Application: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Coating (Coat)

Clone: C80-1608

Product Details anti-CD80 Antibody (CF®594)

Purpose: Mouse Monoclonal anti-CD80 (C80/1608), CF594 Conjugate

Characteristics: T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells and bind the homologous T cell receptors CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and CD28 and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31 % overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counter receptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length CD80 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence 1-2 μg/mL

• Flow Cytometry 0.5-1 μg/million cells
• ELISA For coating, order Ab without BSA
• Optimal dilution for a specific application should be determined by user

Comment:

Raji or Ramos Cells. Tonsil, Spleen or Thymus

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD80

Target: CD80

Alternative Name: CD80

Molecular Weight: 60 kDa

Gene ID: 941, 838

UniProt: P33681

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints