VCAM1 antibody (CF®568)
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- Target See all VCAM1 Antibodies
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This VCAM1 antibody is conjugated to CF®568
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-CD106 / VCAM1 (1.4C3), CF568 Conjugate
- Characteristics
- This antibody recognizes a protein of 110 kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Stimulated human umbilical vein endothelial cells (HUVEC)
- Clone
- 1-4C3
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product VCAM1 Primary Antibody
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- Application Notes
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Human placenta or tonsil
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- Alternative Name
- CD106 / VCAM1 (VCAM1 Products)
- Synonyms
- CD106 antibody, INCAM-100 antibody, Vcam-1 antibody, VCAM1B antibody, fi76d06 antibody, wu:fi76d06 antibody, zgc:158875 antibody, VCAM1 antibody, vascular cell adhesion molecule 1 antibody, vascular cell adhesion molecule 1b antibody, VCAM1 antibody, Vcam1 antibody, vcam1b antibody, VCAM-1 antibody
- Background
- CD106, INCAM-100, L1CAM, Vascular Cell Adhesion Molecule 1 (VCAM1), Vascular cell adhesion protein 1
- Molecular Weight
- ~110 kDa
- Gene ID
- 7412, 109225
- UniProt
- P19320
- Pathways
- Carbohydrate Homeostasis
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