SELPLG antibody (CF®594)

Catalog No. ABIN6157961

This anti-SELPLG antibody is a Mouse Monoclonal antibody detecting SELPLG in FACS, IHC, IF and ISt. Suitable for Human.

Quick Overview for SELPLG antibody (CF®594) (ABIN6157961)

Target: SELPLG (Selectin P Ligand (SELPLG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SELPLG antibody is conjugated to CF®594

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt)

Clone: PSGL1-1601

Product Details anti-SELPLG Antibody (CF®594)

Purpose: Mouse Monoclonal anti-CD162 (PSGL1/1601), CF594 Conjugate

Characteristics: CD162 glycoprotein functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length SELPLG (CD162) protein

Isotype: IgG1

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Jurkat cells. Tonsil or Spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for SELPLG

Target: SELPLG (Selectin P Ligand (SELPLG))

Alternative Name: CD162

Background: CD162, CLA, Cutaneous lymphocyte associated antigen, P-selectin glycoprotein lig, 1, PSGL1, Selectin P lig, SELPLG

Molecular Weight: 43-120 kDa

Gene ID: 6404, 591014

UniProt: Q14242