NGFR antibody (CF®647)

Catalog No. ABIN6158363

This anti-NGFR antibody is a Mouse Monoclonal antibody detecting NGFR in FACS, IF and IHC (f). Suitable for Human, Rabbit, Cat, Ferret, Monkey and Baboon.

Quick Overview for NGFR antibody (CF®647) (ABIN6158363)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human, Rabbit, Cat, Ferret, Monkey, Baboon

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: NGFR5

Product Details anti-NGFR Antibody (CF®647)

Purpose: Mouse Monoclonal anti-NGFR (Nerve-Growth Factor Receptor) (NGFR5), CF647 Conjugate

Characteristics: This antibody recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: NGFR from A875 melanoma cells

Isotype: IgG1 kappa

Application Details

Application Notes: Does not react with mouse or rat, others not known

• Immunohistology formalin-fixed 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR (Nerve-Growth Factor Receptor)

Molecular Weight: 75 kDa

Gene ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding