Calnexin antibody (N-Term) (CF®647)

Catalog No. ABIN6161441

This anti-Calnexin antibody is a Mouse Monoclonal antibody detecting Calnexin in WB, IHC, IF, FACS and ISt. Suitable for Human.

Quick Overview for Calnexin antibody (N-Term) (CF®647) (ABIN6161441)

Target: Calnexin (CANX)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calnexin antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt)

Clone: CANX-1541

Product Details anti-Calnexin Antibody (CF®647)

Binding Specificity: N-Term

Purpose: Mouse Monoclonal anti-Calnexin (CANX/1541), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant N-terminal fragment of human Calnexin protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin

Background: Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90

Molecular Weight: 90 kDa

Gene ID: 821, 567968

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis