Desmoglein 3 antibody (CF®405S)
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- Target See all Desmoglein 3 (DSG3) Antibodies
- Desmoglein 3 (DSG3)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Desmoglein 3 antibody is conjugated to CF®405S
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)
- Purpose
- Mouse Monoclonal anti-Desmoglein-3 (5G11), CF405S Conjugate
- Characteristics
- This antibody recognizes a protein of 130 kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human DSG3 extracellular protein fragment (exact sequence is proprietary)
- Clone
- 5G11
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product DSG3 Primary Antibody
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- Application Notes
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Immunohistology (formalin) 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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A431 cells. Lung squamous cell carcinoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Desmoglein 3 (DSG3)
- Alternative Name
- Desmoglein-3 (DSG3 Products)
- Synonyms
- DSG3 antibody, CDHF6 antibody, PVA antibody, bal antibody, balding antibody, desmoglein 3 antibody, DSG3 antibody, Dsg3 antibody
- Background
- 130 kDa pemphigus vulgaris antigen (PVA), Balding (Bal), Cadherin family member 6 (CDHF6), Desmoglein-3 (DSG3)
- Molecular Weight
- 130 kDa
- Gene ID
- 1830, 1925
- UniProt
- P32926
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