Glypican 3 antibody (AA 511-580) (CF®405S)

Catalog No. ABIN6163675

The Mouse Monoclonal anti-Glypican 3 antibody has been validated for IF, FACS and IHC (f). It is suitable to detect Glypican 3 in samples from Human.

Quick Overview for Glypican 3 antibody (AA 511-580) (CF®405S) (ABIN6163675)

Target: Glypican 3 (GPC3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glypican 3 antibody is conjugated to CF®405S

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 1G12

Product Details anti-Glypican 3 Antibody (CF®405S)

Binding Specificity: AA 511-580

Purpose: Mouse Monoclonal anti-Glypican-3 (1G12), CF405S Conjugate

Characteristics: This antibody recognizes a protein, which is identified as HGAL. It contains a putative PDZ-interacting domain, an immunoreceptor tyrosine-based activation motif (ITAM), and two putative SH2 binding sites. In B cells, its expression is specifically induced by interleukin-4. HGAL is specifically expressed in germinal center B-cells, but is absent in mantle and marginal zone B-cells and in the inter-follicular and para-cortical regions in normal tonsils and lymph nodes. Its high degree of specificity for germinal center B-cells makes anti-HGAL an ideal marker for the detection of germinal center-derived B-cell lymphomas. HGAL expression has been used to help elucidate nodal marginal zone lymphoma (NMZL) from cases of diffuse follicle center lymphoma. Additionally, HGAL expression was shown to correlate with survival in patients with diffuse large B-cell lymphoma (DLBCL). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A recombinant fragment containing amino acids 511-580 of human glypican-3

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

293T cells or Hepatocellular carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: Glypican-3

Background: DGSX, Glypican proteoglycan 3, GPC3, GTR2-2, Heparan sulphate proteoglycan, Intestinal protein OCI-5, MXR7, OCI-5, SDYS, Secreted glypican-3, SGBS1

Molecular Weight: 67 kDa

Gene ID: 2719, 644108

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process