Recombinant Glypican 3 antibody (CF®405S)

Catalog No. ABIN6163789

The Mouse Monoclonal anti-Glypican 3 antibody has been validated for IF, FACS and IHC. It is suitable to detect Glypican 3 in samples from Human and Rat.

Quick Overview for Recombinant Glypican 3 antibody (CF®405S) (ABIN6163789)

Target: Glypican 3 (GPC3)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glypican 3 antibody is conjugated to CF®405S

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: RGPC3-863

Product Details anti-Glypican 3 Antibody (CF®405S)

Purpose: Recombinant Mouse Monoclonal anti-Glypican-3 (rGPC3/863), CF405S Conjugate

Characteristics: Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100 % in follicular carcinoma and 70 % in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human GPC3 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• for 10-20 min followed by cooling at RT for 20 min
•   pH 7.5-8.5
• Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

293T cells or Hepatocellular carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: Glypican-3

Background: DGSX, Glypican proteoglycan 3, GPC3, GTR2-2, Heparan sulphate proteoglycan, Intestinal protein OCI-5, MXR7, OCI-5, SDYS, Secreted glypican-3, SGBS1

Molecular Weight: 67 kDa

Gene ID: 2719, 644108

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process