JUP antibody (Biotin)

Catalog No. ABIN6164958

This Mouse Monoclonal antibody specifically detects JUP in WB, IF, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for JUP antibody (Biotin) (ABIN6164958)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This JUP antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: CTNG-1483

Product Details anti-JUP Antibody (Biotin)

Purpose: Mouse Monoclonal anti-Catenin, gamma (CTNG/1483), Biotin Conjugate

Characteristics: This antibody recognizes a protein of 80-87 kDa, identified as gamma-catenin. The catenins (?, ?, ? and ?) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion. ? T-catenin, also referred to as VR22, is a 895-amino acid protein that is most abundantly expressed in cardio-myocytes and in the peritubular myoid cells of the testis. ? T-catenin binds to ? E-catenin as well as to ?-catenin, and it functions to inhibit Wnt signaling. CTNNA3, the gene that encodes for ?-T-catenin, is located on chromosome 10, and mutations in this gene show a strong correlation to late-onset Alzheimer s disease (LOAD) as well as to dilated cardiomyopathy. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length gamma catenin protein

Isotype: IgG1 kappa

Application Details

Application Notes: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min

• Immunohistology (formalin) 0.1-0.2 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

A431, T47D or MCF-7 cells. Skin

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: Catenin, gamma

Background: ARVD12, Catenin (cadherin-associated protein), gamma 80  kDa, Catenin gamma, CTNNG, Desmoplakin III, Desmoplakin-3, DP3, DPIII, Junction Plakoglobin, PDGB, PKGB

Molecular Weight: 80-87 kDa

Gene ID: 3728, 514174

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location