NADPH Oxidase 4 antibody (CF®405S)
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- Target See all NADPH Oxidase 4 (NOX4) Antibodies
- NADPH Oxidase 4 (NOX4)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This NADPH Oxidase 4 antibody is conjugated to CF®405S
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Application
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat)
- Purpose
- Mouse Monoclonal anti-NOX4 / NADPH-Oxidase-4 (NOX4/1245), CF405S Conjugate
- Characteristics
- The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. NOX4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. It is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human NOX4 protein
- Clone
- NOX4-1245
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product NOX4 Primary Antibody
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- Application Notes
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Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- ELISA For coating, order Ab without BSA
- Optimal dilution for a specific application should be determined by user
- Comment
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PC-12, JAR cells. Kidney
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- NADPH Oxidase 4 (NOX4)
- Alternative Name
- NOX4 / NADPH-Oxidase-4 (NOX4 Products)
- Synonyms
- KOX antibody, KOX-1 antibody, RENOX antibody, AI648021 antibody, nox4 antibody, NOX4 antibody, NADPH oxidase 4 antibody, NOX4 antibody, Nox4 antibody, nox4 antibody, PTRG_09562 antibody
- Background
- Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, KOX-1, NADPH oxidase 4, Nox4, Renal NAD(P)H-oxidase, RENOX
- Molecular Weight
- 70 kDa
- Gene ID
- 50507, 371036
- UniProt
- Q9NPH5
- Pathways
- Carbohydrate Homeostasis, Smooth Muscle Cell Migration
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