Vimentin antibody
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- Target See all Vimentin (VIM) Antibodies
- Vimentin (VIM)
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Reactivity
- Human, Mouse, Rat, Pig, Cow, Cat, Rabbit, Sheep
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Vimentin antibody is un-conjugated
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-Vimentin (LN-6), Purified, with BSA
- Characteristics
- This MAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- Human thymic nuclear extract
- Clone
- LN-6
- Isotype
- IgM
- Top Product
- Discover our top product VIM Primary Antibody
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anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated
anti-Vimentin (VIM) antibodyKO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Vimentin (VIM) (AA 435-466), (C-Term) antibodyVIM Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated
anti-Vimentin (VIM) (AA 430-457), (C-Term) antibodyVIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated
anti-Vimentin (VIM) (AA 2-466) antibodyVIM Reactivity: Human, Mouse, Rat WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Human WB, IHC, StM Host: Rabbit Monoclonal VIM-1937R unconjugated Recombinant Antibody
anti-Vimentin (VIM) (AA 41-140) antibodyVIM Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 3E9 unconjugated
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- Application Notes
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Immunohistology formalin-fixed 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Jurkat cells, Sarcomas, Melanomas
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 200 μg/mL
- Buffer
- PBS/0.05 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- Vimentin (VIM)
- Alternative Name
- Vimentin (VIM Products)
- Background
- VIM
- Molecular Weight
- 57-60 kDa
- Gene ID
- 7431, 455493
- UniProt
- P08670
- Pathways
- Caspase Cascade in Apoptosis
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