Vimentin antibody

Catalog No. ABIN6166864

The Mouse Monoclonal anti-Vimentin antibody is suitable to detect Vimentin in samples from Human, Rat, Mouse, Pig, Cow, Cat, Rabbit and Sheep. It has been validated for IF, FACS and IHC (f).

Quick Overview for Vimentin antibody (ABIN6166864)

Target: Vimentin (VIM)

Reactivity: Human, Rat, Mouse, Pig, Cow, Cat, Rabbit, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: LN-6

Product Details anti-Vimentin Antibody

Purpose: Mouse Monoclonal anti-Vimentin (LN-6), Purified, with BSA

Characteristics: This MAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Human thymic nuclear extract

Isotype: IgM

Application Details

Application Notes: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Jurkat cells, Sarcomas, Melanomas

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: VIM

Molecular Weight: 57-60 kDa

Gene ID: 7431, 455493

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis