Plakophilin 1 antibody (N-Term) (Biotin)

Catalog No. ABIN6166972

The Mouse Monoclonal anti-Plakophilin 1 antibody has been validated for WB, IF and FACS. It is suitable to detect Plakophilin 1 in samples from Human and Mouse.

Quick Overview for Plakophilin 1 antibody (N-Term) (Biotin) (ABIN6166972)

Target: Plakophilin 1 (PKP1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Plakophilin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 10B2

Product Details anti-Plakophilin 1 Antibody (Biotin)

Binding Specificity: N-Term

Purpose: Mouse Monoclonal anti-Plakophilin-1 (10B2), Biotin Conjugate

Characteristics: This antibody recognizes a protein of 75 kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: N-terminus of human Plakophilin-1 protein (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HaCat Cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Plakophilin 1

Target: Plakophilin 1 (PKP1)

Alternative Name: Plakophilin-1

Background: B6P, B, 6 protein, PKP1, Plakophilin-1 (ectodermal dysplasia/skin fragility syndrome)

Molecular Weight: 75 kDa

Gene ID: 5317, 497350

UniProt: Q13835