Progesterone Receptor antibody (CF®647)

Catalog No. ABIN6167103

This Mouse Monoclonal antibody specifically detects Progesterone Receptor in WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Progesterone Receptor antibody (CF®647) (ABIN6167103)

Target: Progesterone Receptor (PGR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Progesterone Receptor antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: PR501

Product Details anti-Progesterone Receptor Antibody (CF®647)

Purpose: Mouse Monoclonal anti-Progesterone Receptor (PR501), CF647 Conjugate

Characteristics: This MAb is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81 kDa) and PR-B (116 kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Progesterone Receptor protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

T47-D Cells or Breast Cancers.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Progesterone Receptor

Target: Progesterone Receptor (PGR)

Alternative Name: Progesterone Receptor

Background: NR3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR, PRA, PRB, Progesterone receptor form A, Progesterone receptor form B

Molecular Weight: PR-A (81 kDa) and PR-B (116 kDa).

Gene ID: 5241, 2905

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration