UCHL1 antibody (CF®488A)

Catalog No. ABIN6168295

The Mouse Monoclonal anti-UCHL1 antibody is suitable to detect UCHL1 in samples from Human, Rat, Mouse, Cow and Pig. It has been validated for WB and IHC (f).

Quick Overview for UCHL1 antibody (CF®488A) (ABIN6168295)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human, Rat, Mouse, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This UCHL1 antibody is conjugated to CF®488A

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 31A3

Product Details anti-UCHL1 Antibody (CF®488A)

Purpose: Mouse Monoclonal anti-PGP 9.5 / UCHL-1 (31A3), CF488A Conjugate

Characteristics: This MAb reacts with a protein of 20-30 kDa, identified as PGP 9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UCHL-1). Initially, PGP 9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP 9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP 9.5 gene is believed to cause a form of Parkinson's disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Native UchL1 (PGP 9.5) protein from brain

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Cerebellum

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: PGP 9.5 (UCHL-1)

Molecular Weight: 20-30 kDa

Gene ID: 7345, 518731

UniProt: P09936

Pathways: Feeding Behaviour