MMP2 antibody (AA 444-575) (CF®647)

Catalog No. ABIN6170354

This anti-MMP2 antibody is a Mouse Monoclonal antibody detecting MMP2 in WB, IF and FACS. Suitable for Human.

Quick Overview for MMP2 antibody (AA 444-575) (CF®647) (ABIN6170354)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MMP2 antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: MMP2-1501

Product Details anti-MMP2 Antibody (CF®647)

Binding Specificity: AA 444-575

Purpose: Mouse Monoclonal anti-MMP2 / Collagenase-Type-IV-A (MMP2/1501), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 72 kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human MMP2 protein fragment (aa444-575) (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

U-138 MG or U-87 MG cells. Placenta

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: MMP2 / Collagenase-Type-IV-A

Background: 72 kD type IV collagenase|CLG4A|Collagenase Type 4 alpha|Collagenase type IV A|Gelatinase A|Gelatinase alpha|Gelatinase neutrophil|Matrix metalloproteinase-2|MMP2|MONA|Neutrophil gelatinase|PEX|TBE-1

Molecular Weight: 72 kDa (Pro), 63 kDa (cleaved)

Gene ID: 4313, 513617

UniProt: P08253

Pathways: Activation of Innate immune Response