Cytokeratin 19 antibody (CF®647)

Catalog No. ABIN6172102

The Mouse Monoclonal anti-Cytokeratin 19 antibody has been validated for IF, FACS and IHC (f). It is suitable to detect Cytokeratin 19 in samples from Human and Mouse.

Quick Overview for Cytokeratin 19 antibody (CF®647) (ABIN6172102)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is conjugated to CF®647

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: KRT19-799 KRT19-800

Product Details anti-Cytokeratin 19 Antibody (CF®647)

Purpose: Mouse Monoclonal anti-Cytokeratin 19 (KRT19/799 + KRT19/800), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 40 kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human KRT19 protein

Isotype: IgG1, IgG2a kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

MCF-7, HeLa cells, Breast Cancer

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: Cytokeratin 19

Background: K19, k1cs, Keratin 19 Keratin Type i 40kD, krt19

Molecular Weight: 40 kDa

Gene ID: 3880, 654568

UniProt: P08727