NEFL antibody (Biotin)

Catalog No. ABIN6172845

This Mouse Monoclonal antibody specifically detects NEFL in IF, FACS and IHC (f). It exhibits reactivity toward Human, Rat, Pig, Cow and Chicken.

Quick Overview for NEFL antibody (Biotin) (ABIN6172845)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human, Rat, Pig, Cow, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFL antibody is conjugated to Biotin

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: NFL-736

Product Details anti-NEFL Antibody (Biotin)

Purpose: Mouse Monoclonal anti-Neurofilament (NF-L) (NFL/736), Biotin Conjugate

Characteristics: This MAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human NEFL protein

Isotype: IgG1

Application Details

Application Notes: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 1-2 μg/mL,Flow Cytometry
• Optimal dilution for a specific application should be determined by user

Comment:

Brain, Neuroblastoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: Neurofilament (NF-L)

Molecular Weight: 68 kDa

Gene ID: 4747, 521461

UniProt: P07196