beta-2 Microglobulin antibody (CF®647)

Catalog No. ABIN6175104

This Mouse Monoclonal antibody specifically detects beta-2 Microglobulin in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for beta-2 Microglobulin antibody (CF®647) (ABIN6175104)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 246-E9-E7|same as HLA-ABC-m2

Product Details anti-beta-2 Microglobulin Antibody (CF®647)

Purpose: Mouse Monoclonal anti-Beta-2 Microglobulin (B2M) (246-E9.E7), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 12 kDa, identified asβ2-microglobulin.β2-microglobulin non-covalently associates with the 44 kDa chain to form the HLA Class I antigen complex. Human β2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. This MAb is specific to human β2-microglobulin and does not react with non-human primate cells. This antibody reacts with all cell types excluding erythrocytes. The detection of β2-microglobulin in body fluids has been used as a tumor marker and for monitoring patients with HIV infection. Clone 246 E9.E7 is the same as HLA.ABC.m2. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human PBLs from a T-cell acute lymphoblastic leukemia (T-ALL) patient

Isotype: IgG2a kappa

Application Details

Application Notes: Does not react with non-human primates, others not known

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: Beta-2 Microglobulin (B2M)

Background: B2M, Beta 2 microglobin, Beta 2 microglobulin, Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, Hdcma22p

Molecular Weight: 12 kDa

Gene ID: 567, 534255

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process