BRCA1 antibody (AA 445-620) (CF®647)

Catalog No. ABIN6175484

This anti-BRCA1 antibody is a Mouse Monoclonal antibody detecting BRCA1 in IF, FACS, ISt and IHC (f). Suitable for Human.

Quick Overview for BRCA1 antibody (AA 445-620) (CF®647) (ABIN6175484)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCA1 antibody is conjugated to CF®647

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: BRCA1-1398

Product Details anti-BRCA1 Antibody (CF®647)

Binding Specificity: AA 445-620

Purpose: Mouse Monoclonal anti-BRCA1 (BRCA1/1398), CF647 Conjugate

Characteristics: The BRCA1 gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human BRCA1 protein fragment (aa445-620) (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes: Immunohistology Formalin-fixed 5-10 μg/mL for 60 min at RT

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer  pH 6.0for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

A431 or HeLa Cells. Breast, Ovarian or Renal Cell Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Molecular Weight: 220 kDa

Gene ID: 672, 194143

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus