MCM7 antibody (AA 195-319) (CF®647)

Catalog No. ABIN6177878

This anti-MCM7 antibody is a Mouse Monoclonal antibody detecting MCM7 in WB, IHC, IF and FACS. Suitable for Human.

Quick Overview for MCM7 antibody (AA 195-319) (CF®647) (ABIN6177878)

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM7 antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: MCM7-1466

Product Details anti-MCM7 Antibody (CF®647)

Binding Specificity: AA 195-319

Purpose: Mouse Monoclonal anti-MCM7 (MCM7/1466), CF647 Conjugate

Characteristics: MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human MCM7 protein fragment (aa195-319) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Raji or Ramos cells. Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Alternative Name: MCM7

Background: CDC47, DNA replication licensing factor MCM7, MCM7 mini chromosome maintenance deficient 7, Mini chromosome Maintenance 7, Mini chromosome maintenance protein 7, P1.1-MCM3, P1CDC47, P85MCM, PNAS146

Molecular Weight: 88 kDa

Gene ID: 4176, 438720

UniProt: P33993

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA