KRT8 antibody

Catalog No. ABIN6179123

This anti-KRT8 antibody is a Mouse Monoclonal antibody detecting KRT8 in IF, FACS and IHC (f). Suitable for Human, Rabbit and Monkey.

Quick Overview for KRT8 antibody (ABIN6179123)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Rabbit, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: KRT8-899

Product Details anti-KRT8 Antibody

Purpose: Mouse Monoclonal anti-Cytokeratin 8 (KRT8/899), Purified, BSA-free

Characteristics: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (?ring-like, perinuclear?) from ductal (?peripheral-predominant?) carcinoma of the breast. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Recombinant human KRT8 protein

Isotype: IgM kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1.0 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background: CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8

Molecular Weight: 52.5 kDa

Gene ID: 3856

UniProt: P05787