TLR4 antibody (Biotin)

Catalog No. ABIN6181205

This anti-TLR4 antibody is a Mouse Monoclonal antibody detecting TLR4 in FACS, IF and IHC (f). Suitable for Human, Rat, Dog, Pig, Guinea Pig and Monkey.

Quick Overview for TLR4 antibody (Biotin) (ABIN6181205)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human, Rat, Dog, Pig, Guinea Pig, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: TLR4-230

Product Details anti-TLR4 Antibody (Biotin)

Purpose: Mouse Monoclonal anti-CD284 / TLR4 (TLR4/230), Biotin Conjugate

Characteristics: This MAb reacts with human Toll-like receptor 2 (TLR4). It is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human TLR4 protein

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa, PC12, human peripheral blood cells. Dendritic cells, macrophage and monocytes in tonsil, placenta and adrenal.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: CD284 / TLR4

Background: ARMD10, CD284, hToll, TLR4, TOLL, Toll Endotoxin Hyporesponsiveness, Toll-like receptor 4

Molecular Weight: 95-120 kDa

Gene ID: 7099, 174312

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins