Recombinant CD15 antibody (CF®405S)

Catalog No. ABIN6183837

This anti-CD15 antibody is a Rabbit Monoclonal antibody detecting CD15 in FACS, IHC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for Recombinant CD15 antibody (CF®405S) (ABIN6183837)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD15 antibody is conjugated to CF®405S

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: FUT4-1478R

Product Details anti-CD15 Antibody (CF®405S)

Purpose: Recombinant Rabbit Monoclonal anti-CD15 / FUT4 (FUT4/1478R), CF405S Conjugate

Characteristics: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95 % of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human neutrophils

Isotype: IgG kappa

Application Details

Application Notes: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min

• Flow cytometry 2-4 μg/million cells
• Immunofluorescence 2-4 μg/mL
• Immunohistology (formalin) 5-10 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

U937 cells, Reed-Sternberg',s cells in Hodgkin',s lymphoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: CD15 / FUT4

Molecular Weight: 220 kDa

Gene ID: 2526, 654379

UniProt: P22083