TRIM29 antibody (AA 1-200) (CF®568)

Catalog No. ABIN6187536

This Mouse Monoclonal antibody specifically detects TRIM29 in IF, FACS and IHC (f). It exhibits reactivity toward Human.

Quick Overview for TRIM29 antibody (AA 1-200) (CF®568) (ABIN6187536)

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIM29 antibody is conjugated to CF®568

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: TRIM29-1041

Product Details anti-TRIM29 Antibody (CF®568)

Binding Specificity: AA 1-200

Purpose: Mouse Monoclonal anti-TRIM29 (TRIM29/1041), CF568 Conjugate

Characteristics: This antibody recognizes a 66 kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90 % positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (126 Amino acid residues between aa 1-200) of human TRIM29 protein

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

A431 cells. Tonsil or Squamous cell carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for TRIM29

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Alternative Name: TRIM29

Background: Ataxia telangiectasia group D complementing gene (ATDC), Tripartite motif-containing protein 29 (TRIM29),

Molecular Weight: 66 kDa

Gene ID: 23650, 504115

UniProt: Q14134

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus