PAX6 antibody (N-Term) (Biotin)

Catalog No. ABIN6189109

The Mouse Monoclonal anti-PAX6 antibody has been validated for WB, IF and FACS. It is suitable to detect PAX6 in samples from Human, Mouse, Rat and Chicken.

Quick Overview for PAX6 antibody (N-Term) (Biotin) (ABIN6189109)

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX6 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PAX6-498

Product Details anti-PAX6 Antibody (Biotin)

Binding Specificity: AA 1-300, N-Term

Purpose: Mouse Monoclonal anti-PAX6 (PAX6/498), Biotin Conjugate

Characteristics: Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (N-terminus, aa 1-300) of human PAX6 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Pancreas or Cerebellum

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: PAX6

Background: AN2, Aniridia type II protein, Oculorhombin, Paired box gene 6 (aniridia keratitis), Paired box homeotic gene 6, Paired box protein Pax-6, Sey, WAGR

Molecular Weight: 47 kDa

Gene ID: 5080, 611376

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis