Melanoma gp100 antibody (CF®405S)

Catalog No. ABIN6189992

This Mouse Monoclonal antibody specifically detects Melanoma gp100 in IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Melanoma gp100 antibody (CF®405S) (ABIN6189992)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: HMB45

Product Details anti-Melanoma gp100 Antibody (CF®405S)

Purpose: Mouse Monoclonal anti-Pmel17 / gp100 / SILV (HMB45), CF405S Conjugate

Characteristics: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Extract of pigmented melanoma metastases from lymph nodes

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Does not react with dog or rat, others not tested
• Optimal dilution for a specific application should be determined by user

Comment:

SK-MEL-28 cells or Melanoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: Pmel17 / gp100 / SILV

Molecular Weight: 90-100 kDa

Gene ID: 6490, 95972

UniProt: P40967