Melanoma gp100 antibody (CF®405S)

Catalog No. ABIN6190030

This anti-Melanoma gp100 antibody is a Mouse Monoclonal antibody detecting Melanoma gp100 in IF, FACS and IHC (f). Suitable for Human and Horse.

Quick Overview for Melanoma gp100 antibody (CF®405S) (ABIN6190030)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is conjugated to CF®405S

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: NKI-beteb

Product Details anti-Melanoma gp100 Antibody (CF®405S)

Purpose: Mouse Monoclonal anti-Pmel17 / gp100 / SILV (NKI-beteb), CF405S Conjugate

Characteristics: By immunohistochemistry this antibody specifically recognizes a in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Membranes from a human melanoma metastasis

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Does not react with rat, others not tested
• Optimal dilution for a specific application should be determined by user

Comment:

SK-MEL-28 cells or Melanoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: Pmel17 / gp100 / SILV

Molecular Weight: 90-100 kDa

Gene ID: 6490, 95972

UniProt: P40967