ACTH antibody (AA 25-39) (CF®640R)
-
- Target See all ACTH Antibodies
- ACTH (Adrenocorticotropic hormone (ACTH))
-
Binding Specificity
- AA 25-39
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This ACTH antibody is conjugated to CF®640R
-
Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Coating (Coat)
- Purpose
- Mouse Monoclonal anti-ACTH (CLIP/1449), CF640R Conjugate
- Characteristics
- ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to CLIP (aa25-39 of ACTH), does not react with Synacthen (aa1-24 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Synthetic peptide corresponding to aa25-39 of human ACTH (NGAEDESAEAFPLEF)
- Clone
- CLIP-1449
- Isotype
- IgG1
- Top Product
- Discover our top product ACTH Primary Antibody
-
-
- Application Notes
-
Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- ELISA For coating, order Ab without BSA
- Optimal dilution for a specific application should be determined by user
- Comment
-
Normal pituitary gland or pituitary tumor
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
-
- Target
- ACTH (Adrenocorticotropic hormone (ACTH))
- Alternative Name
- ACTH (ACTH Products)
- Synonyms
- ACTH antibody, BE antibody, Beta-LPH antibody, Clip antibody, Gamma-LPH antibody, Npp antibody, Pomc-1 antibody, Pomc1 antibody, alpha-MSH antibody, alphaMSH antibody, beta-MSH antibody, gamma-MSH antibody, CLIP antibody, LPH antibody, MSH antibody, NPP antibody, POC antibody, pro-opiomelanocortin-alpha antibody, proopiomelanocortin antibody, Pomc antibody, POMC antibody
- Target Type
- Hormone
- Background
- Adrenocorticotropin, alpha or beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin, beta-Endorphin, beta or gamma Lipotropin (LPH), CLIP, Met Enkephalin, POC, POMC
- Molecular Weight
- ACTH is ~5 kDa
- Gene ID
- 5443, 1897
- UniProt
- P01189
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
-