Recombinant Tyrosinase-Related Protein 1 antibody (CF®488A)

Catalog No. ABIN6191382

The Rabbit Monoclonal anti-Tyrosinase-Related Protein 1 antibody has been validated for IHC, IF and FACS. It is suitable to detect Tyrosinase-Related Protein 1 in samples from Human and Mouse.

Quick Overview for Recombinant Tyrosinase-Related Protein 1 antibody (CF®488A) (ABIN6191382)

Target: Tyrosinase-Related Protein 1 (TYRP1)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Tyrosinase-Related Protein 1 antibody is conjugated to CF®488A

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: TYRP1-1564R

Product Details anti-Tyrosinase-Related Protein 1 Antibody (CF®488A)

Purpose: Recombinant Rabbit Monoclonal anti-Tyrosinase Related Protein-1 (TYRP1/1564R), CF488A Conjugate

Characteristics: This antibody reacts with a 75 kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length TYRP1 protein

Isotype: IgG kappa

Application Details

Application Notes: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min

• Immunohistology (formalin) 5-10 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

SK-MEL-23, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Tyrosinase-Related Protein 1

Target: Tyrosinase-Related Protein 1 (TYRP1)

Alternative Name: Tyrosinase Related Protein-1

Molecular Weight: 75 kDa

Gene ID: 7306, 270279

UniProt: P17643