Biotin antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN619359

The Alkaline Phosphatase (AP)-conjugated Goat Polyclonal anti-Biotin antibody (ABIN619359) specifically detects Biotin.

Quick Overview for Biotin antibody (Alkaline Phosphatase (AP)) (ABIN619359)

Target: Biotin

Host: Goat

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to Alkaline Phosphatase (AP)

Application: Please inquire

Product Details anti-Biotin Antibody (Alkaline Phosphatase (AP))

Purpose: Goat anti-biotin, ALP conjugated

Purification: Immunogen affinity purified

Immunogen: Biotin

Isotype: IgG

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Immunogen affinity purified goat IgG.

Handling Advice: Prepare working dilution prior to use and discard after use. Do not store working dilution

Storage: 4 °C,-20 °C

Storage Comment: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Expiry Date: 48 months

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background:

Background: Goat anti-biotin is a secondary antibody conjugated to ALP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.

Additional information: Purity of this preparation is > 95 % based on SDS-PAGE, Antibody concentration is 1,50 mg/mL (E 1 % at 280 nm = 13,0), Antibody is supplied in 30 mM triethanolamine, pH 7,2,5 mM magnesium chloride, 0,1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative of bacterial growth