Biotin antibody (HRP)

Catalog No. ABIN619361

The Goat Polyclonal anti-Biotin antibody is suitable to detect Biotin.

Quick Overview for Biotin antibody (HRP) (ABIN619361)

Target: Biotin

Host: Goat

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to HRP

Application: Please inquire

Product Details anti-Biotin Antibody (HRP)

Purification: affinity purified

Immunogen: Biotin

Isotype: IgG

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500 - 1:5 000 for Immunohistochemistry 1:200 - 1:5000 for ELISA/Western blot

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handling Advice: Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Storage: 4 °C

Storage Comment: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: Goat anti-biotin is a secondary antibody conjugated to HRP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.