TUBB antibody (Chain beta)

Catalog No. ABIN619536

The Rabbit Polyclonal anti-TUBB antibody (ABIN619536) specifically detects TUBB in WB and IF. The antibody is reactive with Alfalfa, Arabidopsis thaliana and Chlamydomonas reinhardtii samples.

Quick Overview for TUBB antibody (Chain beta) (ABIN619536)

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Alfalfa, Arabidopsis thaliana, Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-TUBB Antibody

Binding Specificity: Chain beta

Purpose: Anti-Tubulin beta chain

Cross-Reactivity (Details): Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa
Not reactive in: Allium sativum

Predicted Reactivity: Brassica napus,Chlamydomonas reinhardii,Glycine max,Hordeum vulgare,Micromonoas pusilla,Nicotiana attenuata,Oryza sativa,Ostreococcus lucimarinus,Picea sitcHensis,Pisum sativum,Physcomitrium patens,Populus trichocarpa,Pythium ultimum,Saccharomyces cerevisiae,Solanum tuberosum,Sorghum bicolor,Ricinus communis,Zea mays,Vigna radiata,Vitis vinifera

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana tubulin beta-2/beta-3 chain P29512

Application Details

Application Notes: 1 : 1000 (IF), 1 : 500 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: Tubulin beta chain

Background:

Background: Tubulin beta (TUB) together with alpha tubulin is making up microtubules.

Additional information: Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 μg/well and a dilution of 1: 500.

Molecular Weight: 49 | 49 kDa (Arabidopsis thaliana)

Gene ID: 836390

UniProt: P29512, P24636, P29513, P29514, P29515, P29516, P29517

Pathways: Microtubule Dynamics, M Phase