ABCB1 antibody (APC)

Catalog No. ABIN6240831

The Mouse Monoclonal anti-ABCB1 antibody has been validated for FACS. It is suitable to detect ABCB1 in samples from Human.

Quick Overview for ABCB1 antibody (APC) (ABIN6240831)

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ABCB1 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: UIC2

Product Details anti-ABCB1 Antibody (APC)

Purpose: Anti-Hu CD243 APC

Specificity: The mouse monoclonal antibody UIC2 recognizes an extracellular epitope on CD243 (MDR-1), an approximately 170 kDa ABC transporter expressed on hematopoietic stem cells, B, T, and NK cells, or on many multidrug resistant cancer cells. This antibody preferentially recognizes CD243 in the process of transporting substrate.

No Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: NIH 3T3 cells transfected with human CD243 (MDR-1) cDNA

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for ABCB1

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Alternative Name: CD243

Background: ATP binding cassette subfamily B member 1,CD243, also known as multidrug resistant protein 1 (MDR-1) or P-glycoprotein (Pgp) is an ATP binding cassette (ABC)-containing efflux transporter for xenobiotic lipophilic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. It is expressed in many tissues, including the brain, liver, pancreas, testes, kidney, and blood (B, T, NK cells, but not monocytes).,P-Glycoprotein, CLCS, MDR1, PGY1, ABC20, GP170, ABCB1, ABC transporter

Gene ID: 5243

UniProt: P08183