DOT1L antibody (C-Term)

Catalog No. ABIN6243177

There are 2+ publications for this product available. The Rabbit Polyclonal anti-DOT1L antibody is suitable to detect DOT1L in samples from Human. It has been validated for IHC (p).

Quick Overview for DOT1L antibody (C-Term) (ABIN6243177)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOT1L antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB2773

Product Details anti-DOT1L Antibody

Binding Specificity: AA 1390-1420, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This KMT4 / Dot1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1390~1420 amino acids from the C-terminal region of human DOT1L.

Isotype: Ig Fraction

Application Details

Application Notes: IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-DOT1L antibody (ABIN6243177)

Nguyen, Xiao, Neppl, Kallin, Li, Chen, Wang, Xiao, Zhang: "DOT1L regulates dystrophin expression and is critical for cardiac function." in: Genes & development, Vol. 25, Issue 3, pp. 263-74, (2011) (PubMed).

Comty, Wathen, Shapiro: "Uremic pericarditis." in: Cardiovascular clinics, Vol. 7, Issue 3, pp. 219-35, (1977) (PubMed).

Target Details for DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Alternative Name: KMT4 / Dot1L

Background: Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger geneRIZ1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ1 is also a tumor susceptibility gene in mice. The loss of RIZ1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ1 promoter strongly correlated with lost or decreased RIZ1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

Molecular Weight: 164856

NCBI Accession: NP_115871

UniProt: Q8TEK3