MLL2 antibody (C-Term)

Catalog No. ABIN6243263

Product Details anti-MLL2 Antibody

Target: MLL2 (Myeloid/lymphoid Or Mixed-Lineage Leukemia 2 (MLL2))

Binding Specificity: AA 4980-5009, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MLL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4980-5009 amino acids from the C-terminal region of human MLL2.

Clone: RB1919-1920

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:80000. WB: 1:27000. IHC-P-Leica: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-MLL2 antibody (ABIN6243263)

Kim, Sharma, Dhar, Lee, Gu, Chan, Lin, Lee: "UTX and MLL4 coordinately regulate transcriptional programs for cell proliferation and invasiveness in breast cancer cells." in: Cancer research, Vol. 74, Issue 6, pp. 1705-17, (2014) (PubMed).

Bhan, Hussain, Ansari, Bobzean, Perrotti, Mandal: "Bisphenol-A and diethylstilbestrol exposure induces the expression of breast cancer associated long noncoding RNA HOTAIR in vitro and in vivo." in: The Journal of steroid biochemistry and molecular biology, Vol. 141, pp. 160-70, (2014) (PubMed).

Dhar, Lee, Kan, Voigt, Ma, Shi, Reinberg, Lee: "Trans-tail regulation of MLL4-catalyzed H3K4 methylation by H4R3 symmetric dimethylation is mediated by a tandem PHD of MLL4." in: Genes & development, Vol. 26, Issue 24, pp. 2749-62, (2012) (PubMed).

Target Details for MLL2

Target: MLL2 (Myeloid/lymphoid Or Mixed-Lineage Leukemia 2 (MLL2))

Alternative Name: MLL2 (MLL2 Products)

Synonyms: AAD10 antibody, ALR antibody, CAGL114 antibody, KABUK1 antibody, KMS antibody, MLL2 antibody, MLL4 antibody, TNRC21 antibody, HRX2 antibody, MLL1B antibody, TRX2 antibody, WBP7 antibody, BC032281 antibody, BC058659 antibody, C430014K11Rik antibody, KMT2B antibody, Mll2 antibody, Mll4 antibody, lysine methyltransferase 2D antibody, lysine methyltransferase 2B antibody, lysine (K)-specific methyltransferase 2D antibody, myeloid/lymphoid or mixed-lineage leukemia 2 antibody, KMT2D antibody, KMT2B antibody, Kmt2d antibody, MLL2 antibody

Background: The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL family, including MLL2. The MLL SET domain is a histone H3 Lys4 (K4)-specific methyltransferase whose activity is stimulated with acetylated H3 peptides. The gene for MLL2 encodes a 5,262-amino acid protein containing a SET domain, 5 PHD fingers, potential zinc fingers, and a long run of glutamines interrupted by hydrophobic residues (mostly leucine). They also detected an alternatively spliced form encoding 4,957 amino acids and lacking an N-terminal zinc finger and PHD finger. By analysis of rodent/human hybrid cells and analysis of the Genebridge radiation hybrid panel, they mapped the gene to the 12p13.1-qter region. The 12q12-q13 region is involved in duplications and translocations associated with cancer. By database searching, Karlin et al. (2002) identified 192 human protein sequences that have multiple amino acid runs, many of which are associated with disease, including cancer. Karlin et al. (2002) found that a key aspect of 82 of these protein sequences is their role in transcription, translation, and developmental regulation. MLL2 is a striking example of proteins with multiple amino acid runs, with 22 glutamine runsGenes encoding a significant number of long amino acid runs are potentially associated with diseases, such as cancer.

Molecular Weight: 593389

NCBI Accession: NP_003473

UniProt: O14686

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Warburg Effect