ALK antibody (C-Term)

Catalog No. ABIN6243474

The Rabbit Polyclonal anti-ALK antibody is suitable to detect ALK in samples from Human. It has been validated for WB.

Quick Overview for ALK antibody (C-Term) (ABIN6243474)

Target: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALK antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB50500

Product Details anti-ALK Antibody

Binding Specificity: AA 1466-1500, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ALK antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1466-1500 amino acids from the C-terminal region of human ALK.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for ALK

Target: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Alternative Name: ALK

Background: Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.

Molecular Weight: 176442

UniProt: Q9UM73

Pathways: RTK Signaling