CHEK1 antibody (C-Term)

Catalog No. ABIN6243849

The Rabbit Polyclonal anti-CHEK1 antibody has been validated for WB. It is suitable to detect CHEK1 in samples from Human and Mouse.

Quick Overview for CHEK1 antibody (C-Term) (ABIN6243849)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB2529

Product Details anti-CHEK1 Antibody

Binding Specificity: AA 425-455, C-Term

Predicted Reactivity: C, Rat, X

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This CHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-455 amino acids from the C-terminal region of human CHK1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHK1

Background: Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. Antibodies against CHK1 recognize a 54-kD protein on immunoblots of mammalian cell extracts, but the protein has altered mobility when isolated from cells treated with ionizing radiation (IR), indicating that CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. Evidence indicates that in response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. Proteolysis of activated CHK1 may promote checkpoint termination under normal conditions and may play a role in the cytotoxic effects of camptothecin and related anticancer drugs.

Molecular Weight: 54434

NCBI Accession: NP_001107593, NP_001107594, NP_001231775, NP_001265

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair