Calnexin antibody

Catalog No. ABIN6251945

This anti-Calnexin antibody is a Mouse Monoclonal antibody detecting Calnexin in WB, IF, FACS and IHC (p). Suitable for Human.

Quick Overview for Calnexin antibody (ABIN6251945)

Target: Calnexin (CANX)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CANX-1541

Product Details anti-Calnexin Antibody

Specificity: Does not react with rat

Purification: Protein G affinity chromatography

Immunogen: A partial human recombinant protein was used as the immunogen for the Calnexin antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: WB: 0.5-1 μg/mL

Flow Cytometry: 0.5-1 μg/10^6 cells

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

Titering of the Calnexin antibody may be required for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin

Background: It recognizes a protein of 90  kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Pathways: MAPK Signaling, Thyroid Hormone Synthesis