Actin, Muscle Specific antibody

Catalog No. ABIN6251982

This Mouse Monoclonal antibody specifically detects Actin, Muscle Specific in FACS, IF and IHC. It exhibits reactivity toward Human, Rabbit and Rat.

Quick Overview for Actin, Muscle Specific antibody (ABIN6251982)

Target: Actin, Muscle Specific

Reactivity: Human, Rabbit, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin, Muscle Specific antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: SPM160

Product Details anti-Actin, Muscle Specific Antibody

Purification: Protein G affinity chromatography

Immunogen: Human myocardium extract was used as the immunogen for this Muscle Specific Actin antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

The optimal dilution of the Muscle Specific Actin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Actin, Muscle Specific

Target: Actin, Muscle Specific

Alternative Name: Muscle Specific Actin

Background: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Gene ID: 59

UniProt: P62736