FOXA1 antibody

Catalog No. ABIN6251996

This anti-FOXA1 antibody is a Mouse Monoclonal antibody detecting FOXA1 in WB, FACS, IF and IHC (p). Suitable for Human and Rat.

Quick Overview for FOXA1 antibody (ABIN6251996)

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXA1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FOXA1-1241

Product Details anti-FOXA1 Antibody

Purification: Protein G affinity chromatography

Immunogen: A human recombinant partial protein corresponding to amin acids 372-472 was used as the immunogen for this FOXA1 antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 0.5-1 μg/mL

WB: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Alternative Name: FOXA1

Background: The transcription factor Forkhead-box A1, also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (alpha and beta), HNF-3 (alpha, beta and gamma), HNF-4 (alpha and gamma), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. Its expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Gene ID: 3169

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis