JUP antibody

Catalog No. ABIN6252060

This Mouse Monoclonal antibody specifically detects JUP in WB, IF and IP. It exhibits reactivity toward Human, Mouse, Rat, Chicken, Cow, Dog and Cat.

Quick Overview for JUP antibody (ABIN6252060)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human, Mouse, Rat, Chicken, Cow, Dog, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 15F11

Product Details anti-JUP Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant full length chicken gamma Catenin was used as the immunogen for the Plakoglobin antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence: 1-2 μg/mL

WB: 0.5-1 μg/mL

Immunoprecipitation: suitable

The stated application concentrations are suggested starting amounts. Titration of the Plakoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: Gamma Catenin

Background: Plakoglobin, or gamma Catenin, is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of Plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). [UniProt]

Gene ID: 3728

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location