ICAM-3/CD50 antibody
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- Target See all ICAM-3/CD50 (ICAM3) Antibodies
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ICAM-3/CD50 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), BioImaging (BI)
- Purification
- Protein G affinity chromatography
- Immunogen
- Stimulated human leukocytes were used as the immunogen for this CD50 antibody.
- Clone
- 186-2G9
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product ICAM3 Primary Antibody
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- Application Notes
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Functional Activity (Blocking): Blocks CD50-CD11a interaction
Flow Cytometry: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD50 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- Alternative Name
- CD50 (ICAM3 Products)
- Synonyms
- CD50 antibody, CDW50 antibody, ICAM-R antibody, ICAM-3 antibody, ICAM3 antibody, intercellular adhesion molecule 3 antibody, ICAM3 antibody, Icam3 antibody
- Background
- This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM3 (Leucocyte Workshop VI). It is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This CD50 antibody is excellent for staining of formalin/paraffin tissues.
- Gene ID
- 3385
- UniProt
- P32942
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